Respiratory Disease Inspection by LED fluorescent microscopy

Respiratory tract infections are the most common clinical disease, caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc.. Atypical respiratory pathogens mostly reply on laboratory method to detect  because of its non-specific clinical symptoms. Immunofluorescence (IF), also known as immune antibody technology is a kind of fluorescein-labeled antibody (antigen) and antigen (antibody) positioning technology. The technology is advantage with no radioactive contamination and easy to operate with strong specificity, high sensitivity and fast inspection. These make it became one of the most commonly used clinical laboratory test methods.

U.S. Diagnostic Hybrids, Inc's respiratory seven JIU kits and Spain Vircell's nine respiratory tract infection pathogens IgM antibody detection kit are the most widely used application respiratory infections immunofluorescence reagent products for detecting respiratory disease . Among them, the United States seven joint inspection of the test items include A-type (influenza) influenza, B-type (type B) influenza, respiratory syncytial virus, adenovirus, parainfluenza virus type 1, 2 and 3, a total of seven kinds of respiratory diseases associated virus. Spain respiratory nine JIU test items include Legionella pneumophila serotype 1, Mycoplasma pneumoniae, Q heat Rickettsia, Chlamydia pneumoniae, adenovirus, respiratory syncytial virus, influenza A virus, influenza B virus, deputy influenza virus type 1, 2 and 3, a total of nine respiratory viruses.

In the respiratory nine and seven JIU most widely used fluorescein reagent is fluorescein isothiocyanate (fluoresceinisothinocyanate, FITC), which presents a bright yellow-green fluorescence under blue light excitation. For fluorescein commonly used in respiratory disease inspection immunofluorescence reagent, Mshot launched LED fluorescence microscope imaging system solution for it, the solution includes LED epi-fluorescence microscope and high sensitivity digital camera (MF10-LED, MF22).

The LED fluorescence illumination can flexibly matched with Olympus, Nikon, Leica and Zeiss microscopes to upgrade them to a energy-efficient, easy operation and long life fluorescent microscope. In the respiratory tract nine JIU applications, Mshot LED fluorescence microscope imaging solutions with superior performance won the U.S. seven JIU, Spain nine JIU reagent users unanimously approved.

Main specification of MF-B-LED fluorescence illumination used on Respiratory Disease Inspection

Excitation color	Excitation filter	Dichroic mirror	Emission filter
Blue	460-490nm	500nm	510nm

Left：Mshot LED fluorescence microscope MF22 

Right: Respiratory Disease Inspection captured performance photo

LED fluorescence illuminator testing report 470nm/450nm

Testing background：Olympus CX22 microscope host, LED fluorescence illuminator in 470bm/450bm, beam spliter head, CCD camera MS31, CCD camera MC20-N Observation fluorescence dye sample：Thermal dye - auramine O - thermal dye rhodamine - dyed auramine O cold - auramine O - dye rhodamine cold - acid - dyed auramine O.

Testing purpose：Main wavelength of mycobacterium tuberculosis in the market is 455nm, standard configuration of our LED fluorescence illuminator is 470nm. The testing is confirm an d prove the difference of those two wavelength, so that fluorescence microscopy users to choose the suitable module.

Firstly, we take use of LED fluorescence illuminator in 450nm match with MS31 camera, fluorescence reagent is Hot dyed auramine O -- rhodamine, below is performance photo

Immunofluorescence by LED Fluorescence Microscope

High quality fluorescence microscope is essential to clinical indirect immunofluorescence diagnostic.Currently, a great amount of fluorescence microscope used at clinical labs are illuminating with mercury lamp. On purpose of improving working efficiency and diagnostic background, Micro-shot company launched a complete solution by LED fluorescence microscope imaging system, which is consist of LED epi-fluorescence microscope and digital camera with analysis software.

Compared to conventional mercury lamp, there are many advantages of LED lighting.We can get to know by below points

a.Specific LED excitation wavelength to excite fluorescence from sample more efficiency. And more friendly to users if without UV excitation.

b.50,000 hours long working life which is 250 times to traditional mercury. Extremely extend working life of microscopes to reduce experiment cost. But contain stable microscopy performance.

c.Real time using without preheating or cooling. Unique LED lighting system to make sure full power supply only it is power on. Open or close freely at any time.

d.More environment friendly without mercury leak, high safety performance, small volume and cold light illuminator is convenience to move.

e.No need of optical path adjustment.
f. Brightness controller can protect fluorescence quenching from exceed high temperature.

g.Available with portable power supplier convenience to use.

Indirect Immunofluorescence Required wavelength :

MSHOT LED Fluorescence Microscopy System :

Mode	LED lighting central wavelength	Excitation filter	Dichroic mirror	Emission filter
Blue (MF-B-LED)	475nm	460-490nm	>500nm	>510nm

When using indirect immunofluorescence to diagnose auto-antibody can achieve different fluorescence karyotype and performance.  MSHOT microscopy especially design and offer professional imaging system and solution for fluorescence diagnostic or observation. Take advantage of high resolution and clarity MSHOT digital cameras , users can acquire high contrast fluorescence photos for recording diagnosing result,typical case study, education, etc.

New ！LED Fluorecent Microscopy

We launched a transformative fluorescence microscopy products - LED Fluorecent Microscopy !

With respect for traditional fluorescent microscope, LED Fluorecent Microscopy using a blue LED excitation light source technology, which improves the performance of the microscope a great extent.

More importantly, LED Fluorecent Microscopy purchase more affordable.

You might be interested in them ：

Epi-fluorescence microscope MF10-LED
Epi-fluorescence microscope MF22
LED Stereo microscope MZX80
LED stereo microscope  MZX81

Welcome to Meet Us on LASER World of PHOTONICS CHINA 2014

Optics grand exhibition ‘LASER World of PHOTONICS CHINA 2014’ will be launched in Shanghai China on March 18-20. Micro-shot company invite all partners and customers to visit our booth to observe and share. We will show our new products and technology.

BOOTH NO.：E1.1562

DATE：MARCH 18-20 2014

TIME：MARCH 18-19  09:00 - 17:00     MARCH 20 09:00 - 16:00

ADDRESS：Shanghai New International Expo Centre (SNIEC)

2345 Longyang Road, Pudong New Area Shanghai P.R.C. 201204

Photonics Website ： http://world-of-photonics.net/en/laser-china/ForVisitors

more info : http://www.m-shot.com/news/140224.html