Respiratory Disease Inspection by LED fluorescent microscopy

Respiratory tract infections are the most common clinical disease, caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc.. Atypical respiratory pathogens mostly reply on laboratory method to detect  because of its non-specific clinical symptoms. Immunofluorescence (IF), also known as immune antibody technology is a kind of fluorescein-labeled antibody (antigen) and antigen (antibody) positioning technology. The technology is advantage with no radioactive contamination and easy to operate with strong specificity, high sensitivity and fast inspection. These make it became one of the most commonly used clinical laboratory test methods.

U.S. Diagnostic Hybrids, Inc's respiratory seven JIU kits and Spain Vircell's nine respiratory tract infection pathogens IgM antibody detection kit are the most widely used application respiratory infections immunofluorescence reagent products for detecting respiratory disease . Among them, the United States seven joint inspection of the test items include A-type (influenza) influenza, B-type (type B) influenza, respiratory syncytial virus, adenovirus, parainfluenza virus type 1, 2 and 3, a total of seven kinds of respiratory diseases associated virus. Spain respiratory nine JIU test items include Legionella pneumophila serotype 1, Mycoplasma pneumoniae, Q heat Rickettsia, Chlamydia pneumoniae, adenovirus, respiratory syncytial virus, influenza A virus, influenza B virus, deputy influenza virus type 1, 2 and 3, a total of nine respiratory viruses.

In the respiratory nine and seven JIU most widely used fluorescein reagent is fluorescein isothiocyanate (fluoresceinisothinocyanate, FITC), which presents a bright yellow-green fluorescence under blue light excitation. For fluorescein commonly used in respiratory disease inspection immunofluorescence reagent, Mshot launched LED fluorescence microscope imaging system solution for it, the solution includes LED epi-fluorescence microscope and high sensitivity digital camera (MF10-LED, MF22).

The LED fluorescence illumination can flexibly matched with Olympus, Nikon, Leica and Zeiss microscopes to upgrade them to a energy-efficient, easy operation and long life fluorescent microscope. In the respiratory tract nine JIU applications, Mshot LED fluorescence microscope imaging solutions with superior performance won the U.S. seven JIU, Spain nine JIU reagent users unanimously approved.

Main specification of MF-B-LED fluorescence illumination used on Respiratory Disease Inspection

Excitation color	Excitation filter	Dichroic mirror	Emission filter
Blue	460-490nm	500nm	510nm

Left：Mshot LED fluorescence microscope MF22 

Right: Respiratory Disease Inspection captured performance photo